منابع مشابه
Studies of Adenovirus Sv40 Hybrid Viruses
E46(+) virus purified by limiting dilution and plaque isolation in AGMK cells consists of a mixture of hybrid and non-hybrid virus particles. Of 102 plaque isolates obtained in HEK cells, 98 contained no detectable hybrid virus, while 4 contained a small proportion of hybrid virus. Plaque isolates from AGMK cells uniformly induced SV40 neoantigen, but when these plaque isolates were plaqued in ...
متن کاملStudies of adenovirus-SV40 hybrid viruses. V. Evidence for linkage between adenovirus and SV40 genetic materials.
Recent studies have established that the adenovirus type 7 (Ad. 7) strain E46+ is a mixed population of infectious adenovirus particles and defective particles consisting of adenovirus capsids containing the segment of SV40 DNA which codes for T antigen.1-4 Because of the inability to obtain the SV40 DNA carriers as a pure population, it has not been possible to determine whether they also carr...
متن کاملIn Vitro Transformation by the Adenovirus-sv40 Hybrid Viruses
Primary weanling hamster kidney cultures were transformed with the adeno 2-SV40, adeno 3-SV40, and adeno 12-SV40 hybrid viruses and with adenovirus type 12. The transformed cell lines which were established were characterized with respect to morphology, virus and antigen content, and chromosome aberrations. The adeno 2 and adeno 3-SV40 hybrid transformed cells had the morphology and T antigen c...
متن کاملEvidence for simian virus 40 (SV40) coding of SV40 T-antigen and the SV40-specific proteins in HeLa cells infected with nondefective adenovirus type 2-SV40 hybrid viruses.
HeLa cells infected with the nondefective adenovirus 2 (Ad2)-simian virus 40 (SV40) hybrid viruses (Ad2(+)ND1, Ad2(+)ND2, Ad2(+)ND4, and Ad2(+)ND5) synthesize SV40-specific proteins ranging in size from 28,000 to 100,000 daltons. By analysis of their methionine-containing tryptic peptides, we demonstrated that all these proteins shared common amino acid sequences. Most methionine-containing try...
متن کاملStudies of adenovirus-SV40 hybrid viruses. IV. An adenovirus type 2 strain carrying the infectious SV40 genome.
Recent studies'-5 have demonstrated that a number of human adenoviruses are capable of forming intergeneric "hybrids," or transcapsidants,5 with SV40 genetic material when propagated in monkey kidney cells naturally or deliberately contaminated with SV40. Such hybrids were recognized by their ability to induce SV40 T antigen detectable by complement fixation (CF) and immunofluorescent (FA) tech...
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ژورنال
عنوان ژورنال: Journal of Virological Methods
سال: 2008
ISSN: 0166-0934
DOI: 10.1016/j.jviromet.2008.02.013